PURIFICATION AND CHARACTERIZATION OF L-ASPARGINASE ENZYME FROM SPINACEA OLERACEA
نویسندگان
چکیده
منابع مشابه
PURIFICATION AND SOME PARTIAL CHARACTERIZATION OF PEROXIDASE ISOENZYME FROM BRASSICA OLERACEA CAPITATA L.
Acetone fractionated peroxidase from crude extract of Brassica oleracea leaves (Cabbage) was purified in three steps on chromatographic columns, using Sp-Sepharose, DEAE-Sepharose and Con A-Sepharose. The specific activity of purified main isoenzyme (BOC-POD) is 1887 u/mg protein with RZ: 3.1, which is 172 times more than the RZ of crude extract with 4.3% recovery. The molecular weight of BOC-P...
متن کاملPurification and Some Partial Characterization of Peroxidase Isoenzyme from Brassica Oleracea Capitata L
Acetone fractionated peroxidase from crude extract of Brassica oleracea leaves (Cabbage) was purified in three steps on chromatographic columns, using Sp-Sepharose, DEAE-Sepharose and Con A-Sepharose. The specific activity of purified main isoenzyme (BOC-POD) is 1887 u/mg protein with RZ: 3.1, which is 172 times more than the RZ of crude extract with 4.3% recovery. The molecular weight of BOC-P...
متن کاملpurification and some partial characterization of peroxidase isoenzyme from brassica oleracea capitata l.
acetone fractionated peroxidase from crude extract of brassica oleracea leaves (cabbage) was purified in three steps on chromatographic columns, using sp-sepharose, deae-sepharose and con a-sepharose. the specific activity of purified main isoenzyme (boc-pod) is 1887 u/mg protein with rz: 3.1, which is 172 times more than the rz of crude extract with 4.3% recovery. the molecular weight of boc-p...
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The snake venom´s thrombin-like enzymes comprise a number of serine proteases, which are functionally and structurally related to thrombin. Purification and partial characterization of a thrombin-like enzyme from the venom of the Iranian snake, Agkistrodon halys, was the aim of this study. Purification was carried out by a combination of variety of chromatographic methods that included: gel...
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A strain of Pseudomonas aeruginosa has been shown to produce an enzyme that lyses viable cells of Staphylococcus aureus. The maximal yield of the enzyme was obtained from shake flask cultures of P. aeruginosa which were grown for 18 to 22 hr at 37 C in Trypticase Soy Broth. A 333-fold purification of the enzyme was obtained by acetone precipitation of the culture liquor, followed by column chro...
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ژورنال
عنوان ژورنال: World Journal of Pharmaceutical Research
سال: 2017
ISSN: 2277-7105
DOI: 10.20959/wjpr201711-9544